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File: 970613_me013_90b_txt_0001.txt
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SUBJECT:  STABILITY OF CRYSTALLINE BOTULINUM NEUROTIXIN TYPE A
IN DISTILLED AND NATICK TAP WATER AT 25C

TREATMENT     TIME         WEIGHT    %RESIDUAL   RESIDUAL     #HUMAN
	             (hours)   PER LD50 (pq)  TOXICITY   TOXIN PQ/ML   MLD/ML(c)

UNDILIUTED(a) 
(140ug/ml)              0              70                        100             1.40x10^8       285.7
                                 1             116                         60.3          8.40X10^7       171.4
                              24           210                          33.3           4.66x10^7       95.0
                              48             481                          14.5          2.03x10^7       41.4
                               72           525                             13.3        1.86x10^7       37.9
                               96           641                           10.9            1.52x10^7       31.0
                             168         1750                           4.0               5.60x10^6       11.4

1:100 (b) 		0	70		100.0		1.40x10^6	2.85       
distilled water  	1	116		 60.3		8.44x10^5	1.72
			24	245		28.5		3.99X10^5	0.81
			48	350		20.0		2.80x10^5	0.57
			72	461		15.1		2.11X10^5	0.43
			96	569		12.3		1.72X10^5	0.35
			168	2708		2.5		3.50x10^4	0.07


1:100 (b)       		0	70		100.0		1.40x10^6	2.86
Natick tap water	1	175		40.0		5.60x10^5	1.14
			24	525		13.3		1.86x10^5	0.37
			48	641		10.9		1.50x10^5	0.30
			72	1750		4.0		5.60x10^4	0.11
			96	3854		1.8		2.52x10^4	0.05
			168	5429		1.2		1.68x10^4	0.03

a.  Undilitued toxin contained 140ug crystalline toxin in 1.0ml phospate buffer.
b. Contained 14ug crystalline toxin in 9.9ml water, eitherdistilled or tap.
c. One Human MLD is approximately equal to 7000 Mouse LD5O's
d. One Mouse LD5O equals 70pg.


TABLE 4.  STABILITY OF CRYSTALLINE NEUROTOXIN A ON BDU FABRIC AND MRE FOIL.

                                          STABILITY´┐Ża (Days) at
SUPPORT      TOXIN      TEMPERATURE       % RELATIVE HUMIDITY
MATERIAL     (LD50)     (0c)              0%      50%   90% 


BDU            300-b     		 5               14      14    14
                        		 45               14      1     1
or-d               62x103-c	    5             14      14    14
MRE                	        45             14      14    14     

a. - Toxicity was measured by the mouse assay.
b-After recovery from the material 7 LD50 units were initiallyavailable for the mouse assay.
c-After recovery from the material 10,503 LD50 units were initiallyavailable for the mouse assay.
d-Results apply to both BDU and MRE.






TABLE 5. EFFECTIVE DETOXICANT OF CRYSTALLINE NEUROTOXIN A.

                                        EXPOSURE TEMPERATURE
                 EXPOSURE TIME               ( 0c)
COMPOUND            MINUTES              5     25          45
                                        (% residual toxin)
                            
CLOROX               
2.63%; pH 10.6         10                NDa    NRTb 
0.13%; pH 9.95          2                NRT    NRT        NRT
0.026%; pH 9.7          2                0.02   NRT        NRT

HYDROGEN PEROXIDE      10                0.005   NRT        NRT
(1.5%; pH 11.2)        20                0.006
                       60                NRT  

ISOPROPYL ALCOHOL      2                         0.013      NRT
(35%,pH 8.6)           10                11      NRT
                       20                14
                       60                1.3

ROCCAL                 10                1.3      NRT         NRT
(0.039%; pH 7.8 )      20                1.5 
                       60                0.02

ROCCAL                 2                 0.16     0.01        NRT
(0.039%; PH 11.1)    10                +c		+	NRT
                      		30                  +       NRT

a Not done.
b NRT=No Residual Toxin.  One LD50=70 pg of crystalline neurotoxinexcept for Roccal (pH 11.1) for which a preparation equal to 135 pgwas used.
c A small number of mice survived when inoculated with theundiluted sample.










                      INEFFECTIVE DETOXIFYING AGENTS a



2% SODIUM BICARBONATE pH 8.9

10% SODIUM SULFITE pH 10.2

0.8% LYSOL pH 7.9

0.6% FORMALIN pH 6.2

3% AMMONIUM HYDROXIDE pH 11.1


0.8M GLYCINE BUFFER, pH 11.1 DESTROYED 98% OF TOXICITY IN 10 MIN AT22C


a CRYSTALLINE NEUROTOXIN A      

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